Academia Open
Vol 9 No 2 (2024): December
Clinical Research

Study of Some Apoptotic Protein Expration in Human Mesenchymal Stem Cells During Toxoplasma Gondii Infection
Studi Ekspresi Beberapa Protein Apoptosis pada Sel Punca Mesenkim Manusia Selama Infeksi Toxoplasma Gondii


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  • Suhair Sameer Jasim*,
  • Safa Yassin Mohammed,
  • Suha Sulaiman Erzayek,
  • Rand Hatem Hussein,
  • Ridhab Ajeel Jasim
Suhair Sameer Jasim
Iraqi ministry of health, Iraq *
Safa Yassin Mohammed
Pure science / University of Anbar, Iraq
Suha Sulaiman Erzayek
College of Pure science / University of Anbar, Iraq
Rand Hatem Hussein
College of Pure science / University of Anbar, Iraq
Ridhab Ajeel Jasim
College of Pure science / University of Anbar, Iraq

(*) Corresponding Author
Picture in here are illustration from public domain image or provided by the author, as part of their works
DOI: https://doi.org/10.21070/acopen.9.2024.10302
Published October 15, 2024
Keywords
  • Toxoplasma gondii,
  • apoptosis,
  • Wharton’s Jelly mesenchymal stem cells,
  • pregnancy,
  • gene expression
How to Cite
Jasim, S. S., Mohammed, S. Y., Erzayek, S. S., Hussein, R. H., & Jasim, R. A. (2024). Study of Some Apoptotic Protein Expration in Human Mesenchymal Stem Cells During Toxoplasma Gondii Infection. Academia Open, 9(2), 10.21070/acopen.9.2024.10302. https://doi.org/10.21070/acopen.9.2024.10302

Copyright (c) 2024 Suhair Sameer Jasim, Safa Yassin Mohammed, Suha Sulaiman Erzayek, Rand Hatem Hussein, Ridhab Ajeel Jasim

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background: Toxoplasma gondii (T. gondii) infection is a significant health concern, particularly during pregnancy, as it can lead to fetal harm and birth abnormalities. Specific Background: The role of apoptosis in managing T. gondii infection remains poorly understood, particularly regarding its molecular mechanisms. Knowledge Gap: The impact of T. gondii infection on apoptosis in mesenchymal stem cells (MSCs) derived from human umbilical cords has not been thoroughly studied in existing literature. Aims: This study aimed to investigate the activation of apoptosis and its regulatory mechanisms in human Wharton’s Jelly mesenchymal stem cells (WJ-MSCs) during T. gondii infection. Results: Using non-enzymatic techniques, we isolated T. gondii from infected and aborted phase-specific placentas. Our findings demonstrated a significant increase in the expression of apoptosis-activating genes (CASP2, CASP3, Bak1) in WJ-MSCs following infection, with a marked decrease in cell viability observed within 2 to 4 hours of exposure to the parasite (P≤0.05). Novelty: This study provides novel insights into the relationship between T. gondii infection and apoptosis in WJ-MSCs, highlighting the specific gene expression changes that occur in response to infection. Implications: Research on T. gondii's apoptotic pathways is crucial for developing therapeutic strategies to mitigate pregnancy-related adverse effects and improve maternal and fetal health outcomes.

Highlights:

 

  1. Increased Apoptosis: T. gondii enhances apoptosis in mesenchymal stem cells.
  2. Cell Viability Impact: Significant decrease in WJ-MSC viability after infection.
  3. Clinical Relevance: Insights can inform strategies to reduce fetal infection risks.

 

Keywords: Toxoplasma gondii, apoptosis, Wharton’s Jelly mesenchymal stem cells, pregnancy, gene expression

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